A web forum for questions and answers (hopefully!) regarding the Suicide Polymerase Endonuclease Restriction (SuPER) methodology.

Thursday, August 11, 2005

Another Restriction Enzyme for SuPER PCR

Dr. Stella Loke, from the University of Sydney, emailed me to tell me that the restriction enzmye TaqIa (TCGA) is superior to Tsp509I. The optimum temperature for the enzmye is 65 degrees, but apparently it can be fully heat inactivated, unlike Tsp509I. This may help avoid the step of adding Proteinase K to the reaction to remove the Tsp509I enzmye.


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