Another Restriction Enzyme for SuPER PCR

Dr. Stella Loke, from the University of Sydney, emailed me to tell me that the restriction enzmye TaqIa (TCGA) is superior to Tsp509I. The optimum temperature for the enzmye is 65 degrees, but apparently it can be fully heat inactivated, unlike Tsp509I. This may help avoid the step of adding Proteinase K to the reaction to remove the Tsp509I enzmye.

SuPER PCR

This website is maintained by the authors of the paper "Suicide polymerase endonuclease restriction, a novel technique for enhancing PCR amplification of minor DNA templates". We are hoping to maintain an open forum for people interested in the technique and to offer any assistance, if possible. We would also like to hear people's comments and maintain a list of restriction enzymes and primers that researchers have used successfully.

Please feel to contact either Stefan Green (sjgreen@fsu.edu) or Dror Minz (mail: minz@volcani.agri.gov.il) or leave a message on this site.

Green, S.J. and Minz, D. 2005. Suicide polymerase endonuclease restriction, a novel technique for enhancing PCR amplification of minor DNA templates. Appl. Environ. Microbiol. 71(8):4721-4727.